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PCR-based detection of Acarapis mites in Iranian honey bee Apis mellifera meda (Hymenoptera: Apidae) | ||
| Journal of Advances in Plant Protection | ||
| دوره 1، شماره 1، شهریور 2024، صفحه 17-22 اصل مقاله (868.53 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22103/japp.2024.23213.1003 | ||
| نویسندگان | ||
| Zahra Hajializadeh1؛ Mahdieh Asadi* 2؛ Mehdi Mansouri3 | ||
| 1Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran, | ||
| 2Department of Plant Protection College of Agriculture Shahid BahonarUniversity of Kerman Kerman -Iran | ||
| 3Department of Agricultural Biotechnology, College of Agriculture, Shahid Bahonar University, Kerman, Kerman, Iran. | ||
| چکیده | ||
| Acarapis mites are one of the world’s most important honey bee pests. A polymerase chain reaction (PCR) was used to amplify the mitochondrial cytochrome oxidase I gene to investigate the infestation of Iranian honey bee Apis mellifera meda populations with Acarapis species. From 2020 to 2022, bee workers were collected from apiaries across nine country provinces. The PCR assays were performed with four primer sets, and Acarapis-positive samples were sequenced to identify species. Acarapis woodi showed the highest infection rate, while A. dorsalis was less, and no infection with A. externus was observed. Different sets of primers were used in this investigation. Two primer sets showed different results: the prevalence of A. woodi ranged from 58.33 to 75% with primer set ACR2 and 25 to 50% with primer set ACR1. This is the first report on the existence of Acarapis mites in Iranian A. mellifera based on molecular techniques. | ||
| کلیدواژهها | ||
| Tracheal mites؛ Honey bee؛ COI؛ Primer | ||
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آمار تعداد مشاهده مقاله: 193 تعداد دریافت فایل اصل مقاله: 114 |
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