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Muhaghegh-Dolatabady, M., Habibizad, J., Bahreini Behzadi, M. R.. (1392). Association of TG-repeats in the 5’-flanking region of bovine growth hormone receptor (GHR) gene with milk production traits and somatic cell count in Holstein cattle. , 1(1), 29-34. doi: 10.22103/jlst.2013.481
M. Muhaghegh-Dolatabady; J. Habibizad; M. R. Bahreini Behzadi. "Association of TG-repeats in the 5’-flanking region of bovine growth hormone receptor (GHR) gene with milk production traits and somatic cell count in Holstein cattle". , 1, 1, 1392, 29-34. doi: 10.22103/jlst.2013.481
Muhaghegh-Dolatabady, M., Habibizad, J., Bahreini Behzadi, M. R.. (1392). 'Association of TG-repeats in the 5’-flanking region of bovine growth hormone receptor (GHR) gene with milk production traits and somatic cell count in Holstein cattle', , 1(1), pp. 29-34. doi: 10.22103/jlst.2013.481
Muhaghegh-Dolatabady, M., Habibizad, J., Bahreini Behzadi, M. R.. Association of TG-repeats in the 5’-flanking region of bovine growth hormone receptor (GHR) gene with milk production traits and somatic cell count in Holstein cattle. , 1392; 1(1): 29-34. doi: 10.22103/jlst.2013.481

Association of TG-repeats in the 5’-flanking region of bovine growth hormone receptor (GHR) gene with milk production traits and somatic cell count in Holstein cattle

Journal of Livestock Science and Technologies
مقاله 5، دوره 1، شماره 1، تیر 2013، صفحه 29-34    اصل مقاله (338.39 K)
نوع مقاله: Research Article (Regular Paper)
شناسه دیجیتال (DOI): 10.22103/jlst.2013.481
نویسندگان
M. Muhaghegh-Dolatabady* ؛ J. Habibizad؛ M. R. Bahreini Behzadi
Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran
چکیده
The growth hormone receptor (GHR) is a member of cytokine/hematopoietin family that mediates the biological actions of growth hormone (GH) on target tissues. Therefore, the purpose of this study was to examine the association of TG-repeat polymorphisms in the 5’-flanking region of bovine GHR gene with milk production traits and somatic cell score (SCS) in Holstein cattle of Iran. The part of 5’-flanking region of GHR gene that encompassed TG-repeat was screened by single strand conformation polymorphism (SSCP) method and DNA sequencing. Five hundred eighteen Iranian Holstein cows were genotyped, giving 3 distinct SSCP patterns (A, B, and C). Frequencies of these patterns for the amplified fragment were 0.21, 0.26 and 0.53, respectively. Statistical analysis revealed that TG-repeat had a significant effect on average daily milk production (P < 0.05) and tended to associate with fat and protein percentage. No significant difference was observed between TG-genotypes and SCS. The association identified in the TG-repeat of GHR gene may have potential to serve as candidate genetic marker for marker assisted selection (MAS) in cattle.
کلیدواژه‌ها
GHR gene؛ 5’-flanking region؛ SSCP؛ microsatellite؛ Cattle
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