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Antagonistic Bacillus thuringiensis isolates from citrus rhizosphere effective against Phytophthora nicotianae | ||
Journal of Advances in Plant Protection | ||
دوره 2، شماره 1، شهریور 2025، صفحه 71-78 اصل مقاله (1.06 M) | ||
نوع مقاله: Original Article | ||
شناسه دیجیتال (DOI): 10.22103/japp.2025.25256.1030 | ||
نویسندگان | ||
Mehdi Azadvar* 1؛ Parvin Mirzaee2 | ||
1Head, Plant Protection Department, Kerman Agricultural and Natural Resources Research and Education Center, AREEO, Kerman, Iran | ||
2Horticulture Group, College of Agriculture, Islamic Azad University, Jiroft Branch, Jiroft, Iran | ||
چکیده | ||
Citrus root rot, caused by Phytophthora nicotianae, is one of the most important diseases affecting citrus trees worldwide. This study aimed to isolate, identify, characterize, and evaluate the antagonistic effect of bacilli rhizobacteria isolated from the citrus rhizosphere in Kerman province, Iran, against P. nicotiana. According to the in vitro dual culture bioassays, five out of 67 Bacillus-like isolates showing the highest antagonistic effect were selected and characterized using phenotypic and PCR-based molecular tests for identification. Phenotypic characteristics and nucleotide sequence analyses of the 16S rRNA gene revealed that the isolates were highly similar and belonged to Bacillus thuringiensis. Findings enhance our understanding of the importance and potential role of B. thuringiensis isolates as biocontrol agents against P. nicotianae, offering a promising alternative to chemical fungicides for managing citrus root rot disease in integrated management and sustainable agriculture programs. These five antagonist isolates could be further evaluated in greenhouse and field experiments for their commercial exploitation. | ||
کلیدواژهها | ||
Bacillus؛ Biological control؛ Rhizobacteria, Root rot | ||
آمار تعداد مشاهده مقاله: 29 تعداد دریافت فایل اصل مقاله: 24 |