دانشگاه شهید باهنر کرمان

 آمار

تعداد نشریات26
تعداد شماره‌ها447
تعداد مقالات4,557
تعداد مشاهده مقاله5,380,003
تعداد دریافت فایل اصل مقاله3,580,071
Fadaei, Erfan, Iravani, Hossein, Rayani, Mohammad Hoseein. (1404). Molecular Detection and Genetic Characterization of Anaplasma phagocytophilum in Canine Blood Samples from Kerman, Iran via Real-Time PCR. , (), 1-6. doi: 10.22103/vcbr.2025.24938.1051
Erfan Fadaei; Hossein Iravani; Mohammad Hoseein Rayani. "Molecular Detection and Genetic Characterization of Anaplasma phagocytophilum in Canine Blood Samples from Kerman, Iran via Real-Time PCR". , , , 1404, 1-6. doi: 10.22103/vcbr.2025.24938.1051
Fadaei, Erfan, Iravani, Hossein, Rayani, Mohammad Hoseein. (1404). 'Molecular Detection and Genetic Characterization of Anaplasma phagocytophilum in Canine Blood Samples from Kerman, Iran via Real-Time PCR', , (), pp. 1-6. doi: 10.22103/vcbr.2025.24938.1051
Fadaei, Erfan, Iravani, Hossein, Rayani, Mohammad Hoseein. Molecular Detection and Genetic Characterization of Anaplasma phagocytophilum in Canine Blood Samples from Kerman, Iran via Real-Time PCR. , 1404; (): 1-6. doi: 10.22103/vcbr.2025.24938.1051

Molecular Detection and Genetic Characterization of Anaplasma phagocytophilum in Canine Blood Samples from Kerman, Iran via Real-Time PCR

Veterinary and Comparative Biomedical Research
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 18 تیر 1404    اصل مقاله (677.27 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22103/vcbr.2025.24938.1051
نویسندگان
Erfan Fadaei1؛ Hossein Iravani* 2؛ Mohammad Hoseein Rayani1
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Baft Branch, Baft, Iran
2Department of Basic Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Baft Branch, Baft, Iran
چکیده
Anaplasmosis is an emerging tick-borne disease caused by obligate intracellular bacteria of the genus Anaplasma, capable of infecting both animals and humans. Due to the increasing stray dog population in Kerman city, Iran, and associated zoonotic risks, this study aimed to detect and differentiate Anaplasma species using molecular methods. Fifty dogs were randomly selected from veterinary clinics and city shelters in Kerman, Iran. When necessary, dogs were restrained using standard chemical or mechanical methods. Blood samples were collected and real-time PCR was conducted. For species differentiation, bidirectional nucleotide sequencing was performed using both forward and reverse primers. Multiple sequence alignment and phylogenetic analysis were subsequently performed. Out of 50 samples, three (6%) tested positive by real-time PCR. Nucleotide sequencing confirmed that all positive samples belonged to Anaplasma phagocytophilum. This study demonstrates the presence of Anaplasma phagocytophilum in dogs in Kerman city, Iran, indicating potential zoonotic risks associated with both stray and clinic-admitted animals.
کلیدواژه‌ها
Anaplasma phagocytophilum؛ Real-Time PCR؛ Dog؛ Iran
آمار
تعداد مشاهده مقاله: 12
تعداد دریافت فایل اصل مقاله: 15


سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب