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Soltani, Ahad, Babaei, Homayoon, Kheirandish, Reza, Imani, Masoud, Mohammadi, Pourya. (1404). The Effects of Epigallocatechin-3-Gallate Extract Encapsulated by Bio-Polymeric Alginate-Chitosan Nanoparticles on Testicular Histopathology and Histomorphometry of Mice Following LPS-Induced Acute Endotoxemia. , 2(1), 51-59. doi: 10.22103/vcbr.2025.24696.1041
Ahad Soltani; Homayoon Babaei; Reza Kheirandish; Masoud Imani; Pourya Mohammadi. "The Effects of Epigallocatechin-3-Gallate Extract Encapsulated by Bio-Polymeric Alginate-Chitosan Nanoparticles on Testicular Histopathology and Histomorphometry of Mice Following LPS-Induced Acute Endotoxemia". , 2, 1, 1404, 51-59. doi: 10.22103/vcbr.2025.24696.1041
Soltani, Ahad, Babaei, Homayoon, Kheirandish, Reza, Imani, Masoud, Mohammadi, Pourya. (1404). 'The Effects of Epigallocatechin-3-Gallate Extract Encapsulated by Bio-Polymeric Alginate-Chitosan Nanoparticles on Testicular Histopathology and Histomorphometry of Mice Following LPS-Induced Acute Endotoxemia', , 2(1), pp. 51-59. doi: 10.22103/vcbr.2025.24696.1041
Soltani, Ahad, Babaei, Homayoon, Kheirandish, Reza, Imani, Masoud, Mohammadi, Pourya. The Effects of Epigallocatechin-3-Gallate Extract Encapsulated by Bio-Polymeric Alginate-Chitosan Nanoparticles on Testicular Histopathology and Histomorphometry of Mice Following LPS-Induced Acute Endotoxemia. , 1404; 2(1): 51-59. doi: 10.22103/vcbr.2025.24696.1041

The Effects of Epigallocatechin-3-Gallate Extract Encapsulated by Bio-Polymeric Alginate-Chitosan Nanoparticles on Testicular Histopathology and Histomorphometry of Mice Following LPS-Induced Acute Endotoxemia

Veterinary and Comparative Biomedical Research
مقاله 8، دوره 2، شماره 1، شهریور 2025، صفحه 51-59    اصل مقاله (906.89 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22103/vcbr.2025.24696.1041
نویسندگان
Ahad Soltani1؛ Homayoon Babaei* 1؛ Reza Kheirandish2؛ Masoud Imani1؛ Pourya Mohammadi3
1Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
2Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
3Department of Chemistry, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
چکیده
Endotoxemia is a significant cause of mortality and abnormalities in animals, particularly neonates, within the first 24 hours of life. It is triggered by the release of lipopolysaccharide from the cell wall of gram-negative bacteria into the bloodstream. This study aimed to investigate the impact of epigallocatechin gallate (EGCG) in its simple form and as nanoparticles on the histopathological changes in the testes of NMRI mice induced by endotoxemia. Sixteen male mice were divided into four experimental groups. The control group received no treatment, while the other three groups were induced with acute endotoxemia by intraperitoneal injection of 6750 µg/kg of lipopolysaccharide. Group 3 received EGCG treatment (25 mg/kg) and group 4 received nano-EGCG treatment (25 mg/kg) intra-peritoneally once daily for 28 days. Evaluation of testicular tissue parameters, including spermatogenesis percentage, Johnsen's score, and meiotic index, showed improvement in the EGCG-treated groups, with no significant difference between the simple and nano forms. In conclusion, EGCG administration alleviates the adverse effects of acute endotoxemia on testicular tissue, with no significant difference observed between encapsulated and unencapsulated EGCG.
کلیدواژه‌ها
Epigallocatechin gallate؛ Endotoxemia؛ Testes؛ Mice
مراجع
1, Rameshrad M, Razavi BM, Hosseinzadeh H. Protective effects of green tea and its main constituents against natural and chemical toxins: A comprehensive review. Food Chem Toxicol. 2017; 100: 115-137.

  1. Raetz CR, Whitfield C. Lipopolysaccharide endotoxins. Annu Rev Biochem. 2002; 71: 635-700.
  2. Moresco EM, LaVine D, Beutler B. Toll-like receptors. Curr Biol. 2011; 21: 488-493.
  3. Deb K, Chaturvedi MM, Jaiswal YK. Gram-negative bacterial LPS induced poor uterine receptivity and implantation failure in mouse: alterations in IL-1beta expression in the preimplantation embryo and uterine horns. Infect Dis Obstet Gynecol. 2005; 13: 125-133.
  4. Jaiswal YK, Jaiswal MK, Agrawal V, Chaturvedi MM. Bacterial endotoxin (LPS)-induced DNA damage in preimplanting embryonic and uterine cells inhibits implantation. Fertil Steril. 2009; 91: 2095-2103.
  5. Agrawal V, Jaiswal MK, Jaiswal YK. Gonadal and nongonadal FSHR and LHR dysfunction during lipopolysaccharide induced failure of blastocyst implantation in mouse. J Assist Reprod Genet. 2012; 29: 163-173.
  6. Hoshino K, Takeuchi O, Kawai T, Sanjo H, Ogawa T, Takeda y, Takeda K, Akira S. Cutting edge: Toll-like receptor 4 (TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4 as the Lps gene product. J Immunol. 1999; 162: 3749-3752.
  7. Ortega-Cava CF, Ishihara S, Rumi MA, Kawashima K, Ishimura N, Kazumori H, Udagawa J, Kadowaki Y, Kinoshita Y. Strategic compartmentalization of Toll-like receptor 4 in the mouse gut. J Immunol. 2003; 170: 3977-3985.
  8. Kurt-Jones EA, Popova L, Kwinn L, Haynes LM, Jones LP, Tripp RA, Walsh EE, Freeman MW, Golenbock DT, Anderson LJ, Finberg RW. Pattern recognition receptors TLR4 and CD14 mediate response to respiratory syncytial virus. Nat Immunol. 2000; 1: 398-401.
  9. Bromfield JJ, Sheldon IM. Lipopolysaccharide initiates inflammation in bovine granulosa cells via the TLR4 pathway and perturbs oocyte meiotic progression in vitro. Endocrinology. 2011; 152: 5029-5040.
  10. Winnall WR, Muir JA, Hedger MP. Differential responses of epithelial Sertoli cells of the rat testis to Toll-like receptor 2 and 4 ligands: implications for studies of testicular inflammation using bacterial lipopolysaccharides. Innate Immun. 2011; 17: 123-136.
  11. Jafari O, Babaei H, Kheirandish R, Samimi AS, Zahmatkesh A. Histomorphometric evaluation of mice testicular tissue following short- and long-term effects of lipopolysaccharide-induced endotoxemia. Iran J Basic Med Sci. 2018; 21: 47-52.
  12. Shokrizadeh H, Babaei H, Imani M, Kheirandish R. Short- and long-term effects of lipopolysaccharide-induced endotoxemia on mice ovarian tissue: histomorphometrical evaluation. Veterinarski arhiv. 2019; 89: 669-682.
  13. Hsu HY, Wen MH. Lipopolysaccharide-mediated reactive oxygen species and signal transduction in the regulation of interleukin-1 gene expression. J Biol Chem. 2002; 277: 22131-22139.
  14. Bokoch GM. Regulation of the phagocyte respiratory burst by small GTP-binding proteins. Trends Cell Biol. 1995; 5: 109-113.
  15. Sanlioglu S, Williams CM, Samavati L, Butler NS, Wang G, McCray PBJr, Ritchie TC, Hunninghake GW, Zandi E, Engelhardt JF. Lipopolysaccharide induces Rac1-dependent reactive oxygen species formation and coordinates tumor necrosis factor-alpha secretion through IKK regulation of NF-kappa B. J Biol Chem. 2001; 276: 30188-30198.
  16. O'Bryan MK, Schlatt S, Phillips DJ, de Kretser DM, Hedger MP. Bacterial lipopolysaccharide-induced inflammation compromises testicular function at multiple levels in vivo. Endocrinology. 2000; 141: 238-246.
  17. Collodel G, Castellini C, del Vecchio MT, Cardinali R, Geminiani M, Rossi B, Spreafico A, Moretti E. Effect of a bacterial lipopolysaccharide treatment on rabbit testis and ejaculated sperm. Reprod Domest Anim. 2012; 47: 372-378.
  18. Reddy MM, Mahipal SV, Subhashini J, Reddy MC, Roy KR, Reddy GV, Reddy PRK, Reddanna P. Bacterial lipopolysaccharide-induced oxidative stress in the impairment of steroidogenesis and spermatogenesis in rats. Reprod Toxicol. 2006; 22: 493-500.
  19. Kim YS, Kim EK, Jeon NJ, Ryu BI, Hwang JW, Choi Ej, Moon SH, Jeon BT, Park PG. Antioxidant Effect of Taurine-Rich Paroctopus dofleini Extracts Through Inhibiting ROS Production Against LPS-Induced Oxidative Stress In Vitro and In Vivo Model. Adv Exp Med Biol. 2017; 975: 1165-1177.
  20. Khan MS, Muhammad T, Ikram M, Kim MO. (2019) Dietary Supplementation of the Antioxidant Curcumin Halts Systemic LPS-Induced Neuroinflammation-Associated Neurodegeneration and Memory/Synaptic Impairment via the JNK/NF-kappaB/Akt Signaling Pathway in Adult Rats. Oxid Med Cell Longev. 2019; 7860650.
  21. Zhu X, Liu J, Chen S, Xue J, Huang S, Wang y, Chen O. Isoliquiritigenin attenuates lipopolysaccharide-induced cognitive impairment through antioxidant and anti-inflammatory activity. BMC Neurosci. 2019; 20: 41.
23 .Higdon JV, Frei B. Tea catechins and polyphenols: health effects, metabolism, and antioxidant functions. Crit Rev Food Sci Nutr. 2003; 43: 89-143.

  1. Hassan E, Kahilo K, Kamal T, Hassan M, Saleh Elgawish M. The protective effect of epigallocatechin-3-gallate on testicular oxidative stress in lead-induced toxicity mediated by Cyp19 gene / estradiol level. Toxicology. 2019; 422: 76-83.
  2. Argentin G, Divizia M, Cicchetti R. Oxidative Stress, Cytotoxicity, and Genotoxicity Induced by Methyl Parathion in Human Gingival Fibroblasts: Protective Role of Epigallocatechin-3-Gallate. J Toxicol Environ Health A. 2015; 78: 1227-1240.
  3. Auberval N, Dal S, Bietiger W, Seyfritz E, Peluso J, Muller C, Zhao M, Marchioni E, Pinget M, Jeandidier N, Maillard E, Schini-Kerth V, Sigrist S. Oxidative Stress Type Influences the Properties of Antioxidants Containing Polyphenols in RINm5F Beta Cells. Evid Based Complement Alternat Med. 2015; 859048.
  4. Hong Z, Xu Y, Yin JF, Jin J, Jiang Y, Du Q. Improving the effectiveness of (-)-epigallocatechin gallate (EGCG) against rabbit atherosclerosis by EGCG-loaded nanoparticles prepared from chitosan and polyaspartic acid. J Agric Food Chem. 2014; 62: 12603-12609.
  5. Ye JH, Augustin MA. Nano- and micro-particles for delivery of catechins: Physical and biological performance. Crit Rev Food Sci Nutr. 2019; 59: 1563-1579.
  6. Lertsutthiwong R, Rojsitthisak P, Nimmannit U. Preparation of turmeric oil-loaded chitosan-alginate biopolymeric nanocapsules, Mater Sci and Eng. 2009; 29(3): 856-860.
  7. Johnsen SG. Testicular biopsy score count--a method for registration of spermatogenesis in human testes: normal values and results in 335 hypogonadal males. Hormones. 1970; 1: 2-25.
  8. Allen J, Diemer T, Janus P, Hales K, Hales B. Bacterial endotoxin lipopolysaccharide and reactive oxygen species inhibit Leydig cell steroidogenesis via perturbation of mitochondria. Endocrine. 2004; 25: 265–275.
  9. Li Z, Zhang D, He Y, Ding Z, Mao F, Luo T, Zhang X. Lipopolysaccharide compromises human sperm function by reducing intracellular cAMP. Tohoku J Exp Med. 2016; 238: 105–112.
  10. Biswas B, Yenugu S. Antimicrobial responses in the male reproductive tract of lipopolysaccharide challenged rats. Am J Reprod Immunol. 2011; 65: 557–568.
  11. Jung JY, Han CR, Jeong YJ, Kim HJ, Lim HS, Lee KH, Park HO, Oh WM, Kim SH, Kim WJ. Epigallocatechin gallate inhibits nitric oxide-induced apoptosis in rat PC12 cells. Neurosci Lett. 2007; 411: 222–227.
  12. Sinha D, Roy S, Roy M. Antioxidant potential of tea reduces arsenite induced oxidative stress in Swiss albino mice. Food Chem Toxicol. 2010; 48: 1032–1039.
  13. Qiaoli Liu , Yun Qian, Feng Chen, Xiaoming Chen, Zhi Chen, Min Zheng. EGCG attenuates pro-inflammatory cytokines and chemokines production in LPS-stimulated L02 hepatocyte. Acta Biochim Biophys Sin. (Shanghai) 2014; 46(1): 31-39.
  14. Stephan JP, Syed V, Jegou B. Regulation of Sertoli cell IL-1 and IL-6 production in vitro. Mol Cell Endocrinol. 1997; 134: 109–118.
  15. Guazzone VA, Jacobo P, Theas MS, Lustig L. Cytokines and chemokines in testicular inflammation: A brief review. Microsc Res Tech. 2009; 72: 620–628.
39, Motwani SK, Chopra S, Talegaonkar S,Kohli K, Ahmad FJ, Khar RP. Chitosan-sodium alginate nanoparticles as submicroscopic reservoirs for ocular delivery: Formulation, optimization and in vitro characterization. Eur J Pharm Biopharm. 2008; 68: 513–525.

40, Raus RA, Nawawi WMW, Nasaruddin RR. Alginate and alginate composites for

biomedical applications. Asian J Pharm Sci.

2021; 16: 280-306.

  1. Goodarzi A, Khanmohammadi M, Ebrahimi-Barough S, Azami M, Amani A, Baradaran-Rafii A, Lotfi bakhshaiesh N, Ai A, Farzin A, Ai J. Alginate=Based hydrogel containing taurine-loaded chitosan nanoparticles in biomedical application. Arch Neurosci. 2019; 6(2): e86349.
42,  Khaled E, El-Ela FLA, El-nesr KA, Zanaty ML. An Herbal Nanohybrid Formula of Epigallocatechin Gallate-Chitosan-Alginate Efficiently Restrict the sexual dysfunction adverse effect of β-adrenergic antagonist drug: propranolol. J Biomed Nanotechnol. 2020; 16: 505-519.

43.  Ali M, Afzal M, Verma M,  Misra-Bhattacharya S,  Ahmad FJ,  Dinda AK. Improved antifilarial activity of ivermectin in chitosan–alginate nanoparticles against human lymphatic filarial parasite. Brugia malayi Parasitol Res. 2013; 112: 2933–2943

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