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Evaluation of genetic variation in einkorn wheat originated from west Iran using microsatellite markers | ||
| مجله بیوتکنولوژی کشاورزی | ||
| Article 9, Volume 12, Issue 2, September 2020, Pages 183-208 PDF (1.24 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.22103/jab.2020.15516.1212 | ||
| Authors | ||
| Mohammad Mehdi Poursiahbidi1; Kianoosh Cheghamirza* 1; Sohbat Bahraminejad1; Ahmad Arzani; Ali Ashraf mehrabi2 | ||
| 1Department of Agronomy and Plant Breeding, Faculty of Agricultural Science and Engineering, Razi University, Kermanshah, Iran | ||
| 2Associate Professor, Department of Agronomy and Plant Breeding, College of Agriculture, Ilam University, Ilam, Iran | ||
| Abstract | ||
| Objective Genetic diversity in field crops and their wild ancestors plays important role in breeding programs. The aim of this study was to investigate the genetic diversity of einkorn (Triticum monococcum L. ssp. boeoticum) wheat genotypes collected from western parts of Iran using SSR markers. Material and methods In this study, genetic variation of 163 genotypes from 34 populations of einkorn wheat collected from western parts of Iran was investigated using 19 SSR loci developed in the A genome of hexaploid wheat. Results In the investigation of 163 einkorn genotypes using 19 microsatellite loci were generated 151 polymorphic alleles with an average of 7.94 per locus. The content of polymorphic information content (PIC) in the studied einkorn genotypes ranged from 0.64 for Xgwm480-3A locus to 0.89 for Xgwm4-4A locus. The mean content of polymorphic information in the studied genotypes was 0.77. According to the results, Xgwm4-4A, Xgwm610-4A and Xgwm282-7A loci were identified as the most suitable primers for studying genetic diversity and differentiation of einkorn wheat genotypes. The mean of Shannon coefficient of 0.16 indicated moderate variation in genotypes under study. The average percentage of polymorphic gene loci in the 34 studied populations was 36.74 and the average heterozygosity was 0.114. Based on analysis of molecular variance for 34 populations, the variations between and within populations were calculated as 7% and 93%, respectively. Cluster analysis based on Jaccard coefficients and UPGMA algorithm classified the einkorn genotypes into 10 distinct groups. The results of the principal coordinate analysis revealed that the two primary vectors explained 29.33% and 24.01% of the total molecular genetic variance, respectively. Conclusion The results indicated the usefulness of microsatellite markers in identifying and grouping einkorn wheat genotypes, so that the obtained information could be used in breeding projects, germplasm conservation planning and collection of einkorn wheat populations. | ||
| Keywords | ||
| Einkorn wheat; Microsatellite markers; Triticum monococcum L. ssp. boeticum; West of Iran | ||
| References | ||
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پهلوانی سمیه، ایزانلو علی، پارسا سهیل، قادری محمدقادر (1395) ارتباط بین صفات کیفی دانه و نشانگرهای مولکولی SSR در برخی از ژنوتیپ‌های گندم نان. پژوهشنامه اصلاح گیاهان زراعی 8 (19)، 36-25. خارستانی هادی، نصراله نژادقمی علی‌اصغر، مهرابی علی‌اشرف (1292) بررسی تنوع ژنتیکی گندم‌های اینکورن با استفاده از نشانگرهای ریزماهواره. مجله الکترونیک تولید گیاهان زراعی 6 (2)، 16-1. زرگانی مهدی؛ رنجبر غلامعلی؛ ابراهیم‌نژاد شاهپور (1394) بررسی مولکولی تنوع ژنتیکی درلاین‌های دابل هاپلوئید گندم نان با استفاده از نشانگرهای SSR. پژوهشنامه اصلاح گیاهان زراعی 7 (15)، 95-88 . عبدالهی سیسی نیر؛ محمدی سیدابوالقاسم؛ علوی‌کیا سید سیامک؛ صادق‌زاده بهزاد (1391) کاربرد نشانگرهای EST-SSR در تعیین ساختار ژنتیکی توده‌های بومی و ارقام اصلاح شده جو و تمایز آنها. سومین همایش ملی بیوتکنولوژی کشاورزی ، مشهد، ایران. قاسمی نسرین؛ میرفخرایی رضاقلی؛ و عباسی علیرضا (1398) بررسی تنوع ژنتیکی ارقام گندم نان (Triticum aestivum L.) با استفاده از نشانگرهای ریزماهواره. پژوهشنامه اصلاح گیاهان زراعی، 11 (29)، 9-16. کافی هانیه، نوابپور سعید، زینلینژاد خلیل، پهلوانی محمدهادی (1397) ارزیابی تنوع ژنتیکی ژنوتیپ‌های گندم نان ایرانی و خارجی با استفاده از نشانگرهای SSR. ژنتیک نوین 13 (2)، 311-307. کوهستانی محمد؛ صادق‌زاده بهزاد؛ ابراهیمی محمدعلی؛ یوسفی ولی‌اله (1395) شناسایی نشانگرهای SSR پیوسته با صفات زراعی در گندم دوروم. دومین کنگره بین المللی و چهاردهمین کنگره ملی ژنتیک ایران 3-1 خرداد، تهران؛ ایران. محمدی مجید؛ میرفخرایی رضاقلی؛ عباسی علیرضا (1392) مطالعه تنوع ژنتیکی گندم نان (.Triticum aestivum L) به کمک نشانگرهای ریزماهواره و تجزیه ارتباطی برای صفات فیزیولوژیک تحت تنش سرمای بهاره .ژنتیک نوین 3، 279-288. نظری مریم؛ و عبدالشاهی روح‌اله (1393) بررسی تنوع ژنتیکی ارقام گندم نان (Triticum aestivum L.) از طریق صفات مورفوفیزیولوژیک و نشانگرهای مولکولی SSR. مجله بیوتکنولوژی کشاورزی، 6 (3): 231-215. واجد ابراهیمی محمدتقی؛ محمدآبادی محمدرضا؛ اسماعیلی‌زاده علی (1396) بررسی تنوع ژنتیکی چهار نژاد از گوسفندان موجود در ایران با استفاده از نشانگرهای ریزماهواره‌ای. فنآوری زیستی در کشاورزی 16، 59-67. واجدابراهیمی محمدتقی، محمدآبادی محمدرضا، اسمعیلی زاده کشکوئیه علی (1394) بررسی تنوع ژنتیکی پنج جمعیت گوسفند ایرانی با استفاده از نشانگرهای ریزماهواره‌ای. مجله بیوتکنولوژی کشاورزی 7(4)، 158-143. References
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